TALgetter: Difference between revisions
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For running the command line application, Java v1.6 or later is required. | For running the command line application, Java v1.6 or later is required. | ||
The arguments of the command line application have the following meaning: | The arguments of the command line application have the following meaning: | ||
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Revision as of 19:51, 15 August 2012
TALgetter allows you to scan input sequences for putative target sites of a given TAL (transcription activator like) effector as typically expressed by many Xanthomonas bacteria. TALgetter uses a local mixture model, which assumes that the nucleotide at each position of a putative target site may either be determined by the binding specificity of the RVD at that position (if binding occurs at that position) or by the genomic context (if no binding occurs). Binding specificities and importance of the individual RVDs has been trained on known TAL effector - target site pairs. Nucleotide preferences of the genomic context are learned from (putative) promoter sequences of A. thaliana and O. sativa.
Web-application
TALgetter is available as a web-application at galaxy.informatik.uni-halle.de:8976. Here, you can also download a command line application that is easily scriptable.
Download
TALgetter is implemented in Java using Jstacs. Here, can download the Jar of the command line application. In addition, we provide the Jar of the Galaxy web-application for installing it in your local Galaxy server.
Running the command line application
For running the command line application, Java v1.6 or later is required.
The arguments of the command line application have the following meaning:
name | comment | type |
input | Input sequences (The sequences to scan for TAL binding sites, FastA) | String |
tal | TAL sequence (Sequence of RVDs, seperated by '-', default = NI-HD-HD-NG-NN-NK-NK) | String |
fp | First position (First position (counted from 5' end) considered for search, default = 0) | Integer |
do | Downstream offset (Number of positions counted from 3' end that are not considered, default = 0) | Integer |
top | Top N (Limit the number of reported hits in all input sequences to at most N, valid range = [1, 10000], default = 100) | Integer |
pval | PVals (Computation of p-Values, range={NONE, COARSE, FINE}, default = COARSE) | {NONE, COARSE, FINE} |
pthresh | p-Value (Filter the reported hits by a maximum p-Value. A value of 0 or 1 switches off the filter., valid range = [0.0, 1.0], default = 1.0) | Double |
model | Model type (TALgetter is the default model that uses individual binding specificities for each RVD. TALgetter13 uses binding specificities that only depend on amino acid 13, i.e., the second amino acid of the repat.While TALgetter is recommended in most cases, the use of TALgetter13 may be beneficial if you search for target sites of TAL effector with many rare RVDs, for instance YG, HH, or S*., range={TALgetter, TALgetter13}, default = TALgetter) | {TALgetter, TALgetter13} |
train | Training sequences (The sequence to use for training the model, annotated FastA, OPTIONAL) | String |
If, for instance, you want to scan the FastA-file path/to/myPromoters.fa
for the top 100 target sites of the TAL effector Talc, you start TALgetter with
java -jar TALgetter.jar input=path/to/myPromoters.fa tal="NS-NG-NS-HD-NI-NG-NN-NG-HD-NI-NN-N*-NI-NN-HD-NG-NI-NN-N*-HD-NN-NG"